Nav1.8 – HEK 293Cell line
Catalog #: 60508

Background
Nav1.8 is a voltage gated type X, alpha subunit sodium channel which in humans is encoded by
the SCN10A gene. This subtype of voltage-gated sodium channel is expressed in nociceptors
and has been proposed as a target for the development of new analgesics [1]. Mice deficient in
Nav1.8 had deficits in sensing inflammatory pain (initiated by tissue damage/inflammation) and
visceral pain (initiated by damage or injury to internal organs) but not neuropathic pain.

Description
NaV1.8 (Genbank # Q9Y5Y9) is a tetrodotoxin-resistant sodium channel isoform. Its
electrophysiological properties vary depending on the type of the associated beta subunits (in
vitro) [2].

Sequence
A synthetic codon-optimized DNA sequence encoding human NaV1.8 protein [3] with C-terminal
Streptavidin-Binding Peptide (SBP) [4] tag is stably integrated in 293HEK-Trex cells.

Application
• Drug compound screening
• Functional assays
• Efficient antigen for mouse immunization

References
1. Catterall, W.A. et al. Pharmacol. Rev. 57 (4): 397–409 (2005).
2. Cummins, T.R., et al. Pain 131 (3): 243–257 (2007).
3. Muzny, D.M., et al. Nature 440:1194-1198 (2006).
4. Wilson, D.S., et al. Protein Expression and Purification 23 (3): 440–446 (2001).

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