CFTR – HEK 293 Cell Line
Catalog #: 60506

Background
Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is
encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports
chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene
affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis
[1]. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent
respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth
retardation) and elevated sweat electrolytes [2].

Description
The CFTR-HEK293 Cell Line expresses full length, human cystic fibrosis transmembrane
conductance regulator (CFTR) protein (Genbank # P13569). The expression of CFTR is
confirmed by Western blotting.

Sequence
A synthetic codon-optimized DNA sequence encoding human CFTR protein [2] with C-terminal
Streptavidin-Binding Peptide (SBP) [3] tag is stably integrated in 293HEK-Trex cells.

Application
• Drug compound screening
• Functional Assays
• Efficient antigen for mouse immunization

References:
1. Gadsby, D.C., et al. Nature 440 (7083): 477–483 (2006).
2. Hillier, L.W., et al. Nature 424:157-164 (2003).
3. McCann, C. M., et al. BioTechniques 38 (6): 945–952 (2005).

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