Fluorogenic DPP8 Assay Kit
Catalog #: 80208

Size: 96 reactions

DESCRIPTION: Dipeptidyl peptidase-8 (DPP8) is a ubiquitously expressed serine
exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides.
DPP8 shares homology and substrate recognition patterns with DPP4 and like DPP4, is
thought to play a role in T-cell activation and immune function. The Fluorogenic DPP8
Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and
profiling applications. It comes in a convenient 96-well format, with purified DPP8
enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions. The key to
the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one
simple step on a microtiter plate is required for DPP8 reactions. The fluorometric
substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore
that can then be measured using a fluorescence reader.

COMPONENTS:

Note: The AMC standard is included so the researcher can quatitatively determine the
specific activity of the enzyme using the AMC standard as a measure of how much
AMC substrate was cleaved to release free AMC.

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

REFERENCES:
1. Lee, H.J. et al., J. Biol. Chem. 281 (50): 38653-38662 (2006)
2. Yu, D.M. et al., FEBS J. 273 (11): 2447-2460 (2006)

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