Tankyrase-2 Histone Ribosylation Assay Kit
Catalog # 80576
Size: 96 reactions

DESCRIPTION: The Tankyrase-2 Histone Ribosylation Assay Kit is designed to
measure Tankyrase-2 activity for screening and profiling applications. The
Tankyrase-2 Histone Ribosylation Assay Kit comes in a convenient format,
with 8-well strips precoated with histone mixture, the antibody against
poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, TNKS
assay buffer, and purified TNKS2 enzyme for 100 enzyme reactions. The key to the
Tankyrase-2 Histone Ribosylation Assay Kit is a highly specific antibody that recognizes
poly (ADP-ribose) modified histone. With this kit, only three simple steps on a microtiter
plate are required for Tankyrase-2 reaction. First, NAD+ is incubated with a sample
containing assay buffer and enzyme for one hour. Next, primary antibody is added.
Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition
of the HRP substrate to produce chemiluminescence that can then be measured using a
chemiluminescence reader. Note: This kit is also suitable for use with cell extracts, but
non-specific ribosylation activity may be detected.

COMPONENTS:
 

STABILITY: 6 months from date of receipt when stored as directed.

APPLICATIONS: Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications. Note: This kit is also suitable for use with cell extracts, but non-specific ribosylation activity may be detected.

REFERENCE: Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

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