PARP2 Homogeneous Assay Kit
Catalog # 80702

Size: 384 reactions

BACKGROUND: The PARP2 Homogeneous Assay Kit is designed to measure
PARP2 activity for screening and profiling applications. PARP2 is known to catalyze
the NAD-dependent addition of poly(ADP-ribose) to histones.

DESCRIPTION: The PARP2 Homogeneous Assay Kit comes in a convenient
AlphaLISA® format, with biotinylated histone substrate, activated DNA, primary
antibody, PARP assay buffer, and purified PARP2 for 384 enzyme reactions.
The key to the PARP2 Homogeneous Assay Kit is a highly specific antibody that
recognizes PARylated substrate. With this kit, only three simple steps are required
for PARP2 reactions. First, a sample containing PARP2 enzyme is incubated with
activated DNA and biotinylated substrate for one hour. Next, acceptor beads and
primary antibody are added, then donor beads, followed by reading the Alpha-counts.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

CONTRAINDICATIONS: Green and blue dyes that absorb light in the AlphaScreen
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent
singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal
reduction due to the presence of excess biotin and iron in this medium. MEM, which
lacks these components, does not affect AlphaScreen assays.

STABILITY: At least one year from date of receipt when stored as directed.

REFERENCES:
Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

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