PARP2 Chemiluminescent Assay Kit
Catalog # 80552

Size: 96 reactions

DESCRIPTION:  The PARP2 Chemiluminescent Activity Assay Kit is designed to
measure PARP2 activity for screening and profiling applications. PARP2 is known to
catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The PARP2
assay kit comes in a convenient 96-well format, with purified PARP2 enzyme, histone
mixture, activated DNA, and PARP2 assay buffer for 100 enzyme reactions. The key to
the PARP2 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit,
only three simple steps are required for PARP2 reactions. First, histone proteins are
coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with an
assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with
streptavidin-HRP followed by addition of the HRP substrate to produce
chemiluminescence that can then be measured using a chemiluminescence reader.
Note: The kit is also suitable for use with cell extracts, but non-specific PARP activity
may be detected.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: Up to 1 year when stored as recommended.

REFERENCE: Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

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