PARP11 Chemiluminescent Assay Kit
Catalog # 80561

Size: 96 reactions

DESCRIPTION: The PARP11 Chemiluminescent Activity Assay kit is designed to
measure PARP11 activity for screening and profiling applications. PARP11 is known to
catalyze NAD-dependent ribosylation. The PARP11 assay kit comes in a convenient 96-
well format, with purified PARP11 enzyme, histone mixture, and PARP assay buffer for
100 enzyme reactions. The key to the PARP11 Chemiluminescent Activity Assay is the
biotinylated substrate. With this kit, only three simple steps are required for PARP11
reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP
biotinylated substrate is incubated with an assay buffer that contains the PARP11
enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the
HRP substrate to produce chemiluminescence that can then be measured using a
chemiluminescence reader. Note: The kit is also suitable for use with cell extracts, but
non-specific PARP activity may be detected.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: Up to 1 year when stored as recommended.

REFERENCE:
Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

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