PARP10 Chemiluminescent Assay Kit
Catalog #: 80560

Size: 96 reactions

Description:  The PARP10 Chemiluminescent Assay Kit is designed to measure
PARP10 activity using purified recombinant PARP10 enzyme (BPS Cat. #80510).
PARP10 has been shown to catalyze NAD-dependent mono-ribosylation. The PARP10
assay kit comes in a convenient 96-well format, with purified PARP10 enzyme, histone
mixture, and PARP assay buffer for 100 enzyme reactions. The key to the PARP10
Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple
steps are required for PARP10 reactions. First, histone proteins are coated on a 96-well
plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that
contains the PARP10 enzyme. Finally, the plates are treated with streptavidin-HRP
followed by addition of the HRP substrate to produce chemiluminescence that can then
be measured using a chemiluminescence reader.

Components:
 

Applications: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

Stability: Up to 1 year when stored as recommended.

References:
Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
Kleine H, et. al. Molecular Cell 2008 32 :57-69.

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