PARP1 Chemiluminescent Assay Kit
Catalog # 80551

Size: 96 reactions

DESCRIPTION: The PARP1 Chemiluminescent Activity Assay kit is designed to
measure PARP1 activity for screening and profiling applications. PARP1 is
known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones.
The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1
enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100
enzyme reactions. The key to the PARP1 Chemiluminescent Activity Assay is the
biotinylated substrate. With this kit, only three simple steps are required for
PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the
PARP1 biotinylated substrate is incubated with an assay buffer that contains the
PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by
addition of the HRP substrate to produce chemiluminescence that can be
measured using a chemiluminescence reader.
Note: This kit is also suitable for use with cell extracts, but nonspecific
ribosylation activity may be detected.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.

STABILITY: Up to 1 year when stored as recommended.

REFERENCE: Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

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