MLL1 Complex Direct Activity Assay Kit
Catalog # 53008

Size: 96 reactions

DESCRIPTION: The MLL1 Complex Direct Activity Assay kit is designed to measure
MLL1 activity for screening and profiling applications, using purified MLL1 and its
complex components: WDR5, RbBP5,Ash2, and DPY30. The MLL1 Complex Direct
Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with
histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a
secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer,
and purified MLL1 enzyme complex for 100 enzyme reactions. The key to the MLL1
Complex Direct Activity Assay Kit is a highly specific antibody that recognizes
methylated K4 residue of Histone H3. With this kit, only three simple steps on a
microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine
is incubated with a sample containing assay buffer and methyltransferase enzyme for one
hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled
secondary antibody followed by the addition of the HRP substrate to produce
chemiluminescence that can be measured using a chemiluminescence reader.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

STABILITY: Up to one year from date of receipt when stored as directed.

REFERENCE: Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

Click here to download product PDF and Protocol