Histone H3(K9) Universal Methyltransferase Assay Kit - New
Catalog #: 52072

Size: 96 reactions

DESCRIPTION: The Histone H3(K9) Universal Methyltransferase Assay Kit is designed
for the detection of histone H3(K9) methyltransferase (HMT) activity using purified
enzymes or cell extracts. Histone H3(K9) methyltransferases are enzymes that catalyze
the transfer of a methyl group from the cofactor S-adenosylmethionine to lysine 9
residue of histone H3. The Histone H3(K9) Universal Methyltransferase Assay Kit comes
in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate,
the antibody against methylated lysine residue of Histone H3, the secondary HRPlabeled
antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified
G9a, SUV39H1 and SUV39H2 enzymes for 100 enzyme reactions. The key to the
Histone H3(K9) Universal Methyltransferase Assay Kit is a highly specific antibody that
recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on
a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine
is incubated with a sample containing assay buffer and methyltransferase enzyme for one
hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled
secondary antibody followed by addition of the HRP substrate to produce chemiluminescence
that can then be measured using a chemiluminescence reader.

COMPONENTS:

 

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

CONTRAINDICATIONS: DMSO >1%, strong acids or bases, ionic detergents, high salt

STABILITY: Up to 1 year from date of receipt when stored as directed.

REFERENCE: Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

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