EZH2 Homogeneous Assay Kit
Catalog #52059

Size: 384 reactions

DESCRIPTION: The EZH2 Homogeneous Assay Kit is designed to measure activity of
the EZH2 using purified enzyme complex or cell extracts containing EZH2. EZH2 is a
histone methyltransferase that exhibits methylation activity toward H3-K27. The EZH2
Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with histone substrate
mixture (his-tagged), primary antibody, methylation assay buffer, and purified EZH2 for
384 enzyme reactions. The key to the EZH2 Homogeneous Assay Kit is a highly specific
antibody that recognizes methylated substrate. With this kit, only three simple steps on a
microtiter plate are required for methyltransferase detection. First, a sample containing
EZH2 enzyme is incubated with the histone substrate for one hour. Next, acceptor beads
and primary antibody are added, then donor beads, followed by reading the Alpha-counts. 

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
 
CONTRAINDICATIONS:   Green  and blue dyes  that absorb  light  in  the AlphaScreen
signal emission  range  (520-620 nm),  such as Trypan Blue. Avoid  the use of  the potent
singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+  and  Ni2+).  The  presence  of  >1%  RPMI  1640  culture medium  leads  to  a  signal
reduction  due  to  the  presence  of  excess  biotin  and  iron  in  this medium. MEM, which
lacks these components, does not affect AlphaScreen assays.

STABILITY:  At least one year from date of receipt, when stored as directed.

REFERENCES: Dillon SC, et al. 2005. Genome Biology 6:227.  

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