DNMT Universal Assay Kit
Catalog #52035

Size: 96 reactions

DESCRIPTION: The DNMT Universal Assay Kit is designed to measure DNMT activity
using purified enzymes. The DNMT Universal Assay Kit comes in a convenient format,
with a 96-well plate precoated with DNMT substrate, an antibody against 5-
methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT
assay buffer, and purified DNMT1, DNMT3A/3L and DNMT3B/3L for 100 enzyme
reactions. The key to the DNMT Universal Assay Kit is a highly specific antibody that
recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a
microtiter plate are required for detection of DNMT activity. First, S-adenosylmethionine
is incubated with a sample containing assay buffer and DNMT for two hours. Next,
primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary
antibody followed by addition of the HRP substrate to produce chemiluminescence that
can be measured using a chemiluminescence reader. Note: The kit is also suitable for
use with cell extracts, but total DNMT activity will be detected. The kit will not distinguish
the activity of individual DNMT isoforms in whole cell lysates.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
 
CONTRAINDICATIONS: DMSO >1%,  strong acids or bases,  ionic detergents, high salt
 
STABILITY:  One year from date of receipt when stored as directed.
 
REFERENCE: 1. Svedruzic, Z.M. Curr. Med. Chem. 2008; 15(1):92-106.

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