p110α/p85α
Human, recombinant, GST-tag

Catalog #: 40621
Lot#: 100426

Size: 20 µg

Description: Complex of N-terminal GST-tagged
recombinant full-length human p110α
(GenBank Accession No. U79143), and recombinant
full length, human p85α (no tag) (GenBank
Accession No. XM_043865). Coexpressed in a
Baculovirus infected Sf9 cell expression system.
p110α: MW=151 kDa, p85α: MW=88 kDa.

Specific Activity: 756 pmol/min/μg. A 25 μl
kinase reaction is conducted in a buffer containing 40
mM Tris-HCl (pH 7.4), 20 mM MgCl₂, 100 μM
ATP, 200 μM PI:PS (BPS # 40560) and enzyme for 15 
min at 30°C. ATP reduction is detected using ADP-Glo™
Kinase Assay Platform (Promega Corporation,
Madison, WI). 

Application: Useful for the study of
enzyme kinetics, screening inhibitors, and
selectivity profiling.

Formulated in:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4
mM KCl, 18 mM glutathione, 10% glycerol,
and 3 mM DTT.  

Stability: >6 months at –80°C

References:
1. Vogt, PK et al., Virology. 344(1):131-(2006)
2. Bader, AG etal., Nat Rev Cancer. Dec
     5(12):921-9 (2005)

PRODUCT DATA SHEETS
Lot 100426

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