GIPR/CRE Luciferase Reporter HEK293 Cell Line

Catalog #

78589

$11,115 *

Size: 2 vials

Qty

Bulk/Custom

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Description

GIPR/CRE Luciferase Reporter HEK293 Cell Line is an engineered HEK293 cell line expressing firefly luciferase under the control of cAMP response element (CRE), and human GIPR (Gastric Inhibitory Polypeptide receptor; NM_000164.4). Activation of GIPR in these cells can be monitored by measuring luciferase activity.

The functionality of the GIPR/CRE Luciferase Reporter HEK293 Cell Line was validated in dose-response assays using the agonists gastric inhibitory peptide (GIP), Tirzepatide, and Retatrutide. These agonists induce luciferase activity in a dose-dependent manner as depicted in Figure 1.

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Figure 1. Illustration of the mechanism of action in the GIPR/CRE Luciferase Reporter HEK293 Cell Line.

Interested in screening and profiling inhibitors, blocking antibodies, or activators of GIPR without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Gastric inhibitory polypeptide receptor, GIP-R, Glucose-dependent insulinotropic polypeptide receptor, GIPR, GIP responsive cell line, Tirzepatide responsive cell line, Retatrutide responsive cell line

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Product Data Gallery

Cell surface expression of GIPR in the GIPR/CRE Luciferase Reporter HEK293 Cell Line

Dose response curve of GIPR/CRE Luciferase Reporter HEK293 Cell Line to GIP in a 96-well assay format

Dose response curve of GIPR/CRE Luciferase Reporter HEK293 Cell Line to Tirzepatide, a dual peptide agonist of GIPR and GLP-1R, in a 96-well assay format

Dose response curve of GIPR/CRE Luciferase Reporter HEK293 Cell Line to Retatrutide, a triple agonist peptide of GIPR, GLP-1R and GCGR, in a 96-well assay format

Dose response curve of GIPR/CRE Luciferase Reporter HEK293 Cell Line to GIP in a 384-well assay format

Dose response curve of GIPR/CRE Luciferase Reporter HEK293 Cell Line to Tirzepatide, a dual peptide agonist of GIPR and GLP-1R, in a 384-well assay format

Dose response curve of GIPR/CRE Luciferase Reporter HEK293 Cell Line to Retatrutide, a triple agonist peptide of GIPR, GLP-1R and GCGR, in a 384-well assay format

Product Info

Storage and Usage

Citations

Host Cell Line

HEK293, Human Embryonic Kidney, epithelial-like cells, adherent

Supplied As

Each vial contains >1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1G	BPS Bioscience #79544

Materials Required for Cellular Assay

Name	Ordering Information
Gastric Inhibitory Peptide (GIP), human	Genscript #RP10795
Tirzepatide hydrochloride	MedChemExpress #HY-P1731
Retatrutide	MedChemExpress #HY-P3506
Opti-MEM Reduced Serum Medium (Assay Medium)	ThermoFisher #31985-070
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer

UniProt #

P48546

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The gastric inhibitory polypeptide receptor (GIPR), also known as glucose-dependent insulinotropic polypeptide receptor, belongs to the Class B1 G protein-coupled receptor (GPCR) family. GIPR is primarily found in the β-cells of the pancreas and serves as a receptor for the gastric inhibitory polypeptide (GIP) hormone. As one of the incretin hormones, GIP modulates glucose metabolism by stimulating the pancreatic β-cells to release insulin. Since GIPR/GLP-1R heterodimerization regulates GLP-1R signaling, dual agonists that bind both GIPR and GLP-1R have shown promising clinical efficacy for treating type II diabetes mellitus (T2DM) and obesity.

References

Yang B., et al., 2022 Molecular Metabolism 66: 101638

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin.

Growth Medium 1G (BPS Bioscience #79544): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin, 400 µg/ml of Geneticin and 50 µg/ml of Hygromycin B.

Media Required for Functional Cellular Assay
Assay Medium: Opti-MEM reduced serum medium.

Applications

Screen and characterize agonists of human GIPR in a cellular model.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only