LSD1 Homogeneous Assay Kit
Catalog #50108

Size: 384 reactions

DESCRIPTION: The LSD1 Homogeneous Assay Kit is designed to measure activity of
lysine-specific demethylase (LSD1) for screening and profiling applications. LSD1 is a
chromatin-modifying enzyme that specifically removes methyl groups from mono- and
dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation
via epigenetic histone modifications and is therefore a potential target for drug
development. The LSD1 Homogeneous Assay Kit comes in a convenient AlphaLISA®
format, with biotinylated histone H3 peptide substrate, primary antibody, LSD1 assay
buffer, and purified LSD1 for 384 enzyme reactions. The key to the LSD1 Homogeneous
Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this
kit, only three simple steps on a microtiter plate are required for demethylase detection.
First, a sample containing LSD1 enzyme is incubated with the biotinylated substrate. Next,
acceptor beads and primary antibody are added, then donor beads, followed by reading
the Alpha-counts.

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.

CONTRAINDICATIONS: Green and blue dyes that absorb light in the AlphaScreen
signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent
singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal
reduction due to the presence of excess biotin and iron in this medium. MEM, which
lacks these components, does not affect AlphaScreen assays.

STABILITY: At least one year from date of receipt when stored as directed.

REFERENCES:
1. Forneris, F., Binda, C., Dall'Aglio, A., Fraaije, M.W., Battaglioli, E., and Mattevi, A.
J. Biol. Chem. 2006; 281(46):35289-95.
2. Zhou, M., Diwu, Z., Panchuk-Voloshina, N., and Haugland, R.P. Anal. Biochem. 1997;
253(2):162-8.

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