JMJD2C Chemiluminescent Assay Kit
Catalog # 50405

Size: 96 reactions

DESCRIPTION: The JMJD2C Chemiluminescent Assay Kit is designed to measure JMJD2C
activity for screening and profiling applications. JMJD2C is a JmjC-domain protein that
exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3. The JMJD2C Assay Kit
comes in a convenient format, with a 96-well plate* precoated with the methylated histone
H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody,
demethylase assay buffer, and purified JMJD2C for 100 enzyme reactions.

The key to the JMJD2C Chemiluminescent Assay Kit is a highly specific antibody that
recognizes demethylated substrate. With this kit, only three simple steps on a microtiter
plate are required for methyltransferase detection. First, a sample containing JMJD2C
enzyme is incubated with a sample containing assay buffer for one hour. Next, primary
antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody
followed by addition of the HRP substrate to produce chemiluminescence that can then be
measured using a chemiluminescence reader. Note: The kit is also suitable for use with
cell extracts, but non-specific demethylase activity may be detected.
 
*The JMJD2C assay kit is also available in 8-well microstrip format; contact BPS Bioscience
for more information. 

COMPONENTS:

APPLICATIONS: Great for studying enzyme kinetics and HTS applications.
 
CONTRAINDICATIONS: DMSO >1%,  strong acids or bases,  ionic detergents, high salt
 
STABILITY: One year from date of receipt when stored as directed.
 
REFERENCE: Whetstine JR et al. (2006) Cell  125: 467. 

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