TGFβ1, Active
Human, recombinant  
Catalog #: 90900-3

Size: 1000 µg

Description:  Human  TGFb1  (GenBank
Accession #NM_000660), a.a. 279-390(end),
disulfide-linked homodimer. MW= ~25 kDa.
 
Source: FreeStyle 293-F cells.
 
Endotoxin Level: <1 EU/μg.
 
Biological Activity: measured by TGFβ1’s
ability to inhibit mIL-4-dependent
proliferation of HT-2 mouse helper T cells.
The ED50 is <0.05ng/ml.
 
Application: Useful  for  cell  culture  and
for  the  study  of  signaling  and  apoptotic
pathways.

Formulation:  Lyophilized  from  a  0.2  μM
filtered, aqueous solution

Reconstitution: Supplied  as  a  lyophilized
solid.    Reconstitute  in  sterile  0.2 M  acetic  acid
with 0.1% BSA.
 
Stability: ≥12  months  at  –80°C  (as  a  solid). 
Store reconstituted protein in aliquots at –20°C to
–80°C  for  up  to  6  months.  Avoid  freeze/thaw
cycles.
 
References:
1.  Derynck,  R.  et  al.  (1985).  Nature
     316(6030):701-5.
2.  Bourdrel  L.,  et  al.  (1993).  Protein  Expr.
     Purif. 4:130-140.
3.  Munger,  J.S.,  et  al.  (1997).  Kidney  Int.
     51:1376-1382.