EGF (mouse, recombinant)
Catalog #: 90200-3

Size: 1000 µg

Description: Mouse Epidermal Growth Factor (GenBank Accession No. NM_010113), a.a. 977-
1029, MW=6.0 kDa, expressed in an E.coli expression system.

Source: E.coli

Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C.

Purity: >98% by SDS-PAGE and HPLC analysis.

Endotoxin Level: < 1EU per µg of EGF as determined by the LAL method.

Biological Activity: ED50 is 0.1 – 0.2 ng/ml as determined by a cell proliferation assay using
BALB/c 3T3 cells.

References:
1. Suppressor of cytokine signaling-2 limits intestinal growth and enterotrophic actions of
IGF-I in vivo Am. J. Physiol. Gastrointest. Liver Physiol. 291 (3), G472-G481
2. The kinases MSK1 and MSK2 are required for epidermal growth factor-induced, but
not tumor necrosis factor-induced, histone H3 Ser10 phosphorylation J. Biol. Chem. 281
(18), 12521-12525 (2006)