BD-2, Human beta Defensin-2 Recombinant
Catalog # 90107-A
Size: 5 µg

Description:  Recombinant  human  β-
Defensin-2  is  a  disulfide-linked
monomeric  protein  consisting  of  42
amino  acids  and  migrates  as  an
approximately  4  kDa  protein  under
reducing and non-reducing conditions.  

Source:  Optimized  DNA  sequence
encoding  human  β-Defensin-2  mature
chain was expressed in E. coli.

Formulation: Lyophilized from a 0.2 μm
filtered  solution  containing  100  mM
NaCl, 20 mM Phosphate buffer, pH 7.4.

Reconstitution:  Reconstitute at 0.1-1.0
mg/mL  in  distilled  water.  This  solution
can then be diluted into other buffers. To
maximize  product  collection  from  vial
surface,  vortex  briefly  and  then  spin
down to recollect the liquid. 

Storage:  The  lyophilized  protein  is
stable  for  at  least  2  years  from  date  of
receipt  when  stored  at  -20°C. Upon
reconstitution,  store  in working  aliquots
at  2  -  8° C  for  up  to  one month,  or  at      
-20°C  for  up  to  six  months,  in  the
presence  of  a  carrier  protein. Avoid
repeated freeze/thaw cycles.

Purity:  >98%,  as  determined  by  SD-SPAGE
and HPLC.

Endotoxin  Level:  Endotoxin  level  was
found to be < 0.1 ng/μg (1EU/μg), using
the LAL gel clot method.
 
Biological  Activity:  Determined  by  its
ability  to  chemoattract  immature human
dendritic  cells  using  a  concentration
range of 1-50 ng/ml.
 
Protein Sequence: 
GIGDPVTCLKSGAICHPVFCPRRYKQI
GTCGLPGTKCCKKP

References:
1.   J.  Biol.  Chem.,  Apr  2009;  284:
     10034 – 10045.
2.   J.  Immunol., Feb 2009; 182: 1609 –
     1616.
3.  Invest.  Ophthalmol.  Vis.  Sci.,  Apr
     2009; 50: 848.