BCA-1, Human BCA1(CXCL13) Recombinant
Catalog # 90104-B
Size: 20 µg

Description:  Recombinant  human
BCA1  (B  cell-  attracting  chemokine  1),
also  known  as  CXCL13  or  BLC,  is  a
disulfide-linked  monomeric  protein
consisting  of  88  amino  acids  and
migrates  as  an  approximately  10  kDa
protein  under  reducing  and  non-reducing
conditions. Human  CXCL13, generated  by 
the  proteolytic  removal  of the signal peptide
and propeptide, has a calculated molecular
mass of ~10 kDa.

Source:  Optimized  DNA  sequence
encoding  human  BCA1 mature  domain
was expressed in E. coli.

Formulation: Lyophilized from a 0.2 μm
filtered  solution  containing  20  mM
phosphate buffer, pH 7.5, 100 mM NaCl.
 
Reconstitution:  Reconstitute at 0.1-1.0
mg/mL  in  distilled  water.  This  solution
can then be diluted into other buffers. To
maximize  product  collection  from  vial
surface,  vortex  briefly  and  then  spin
down to recollect the liquid. 

Storage:  The  lyophilized  protein  is
stable  for  at  least  2  years  from  date  of
receipt  when  stored  at  -20°C. Upon
reconstitution,  store  in working  aliquots
at  2  -  8° C  for  up  to  one month,  or  at      
-20°C  for  up  to  six  months,  in  the
presence  of  a  carrier  protein. Avoid
repeated freeze/thaw cycles. 

Purity:  >98%,  as  determined  by  SDS-PAGE
and HPLC.

Endotoxin  Level:  Endotoxin  level  was
found to be < 0.1 ng/μg (1EU/μg), using
the LAL gel clot method.
 
Biological  Activity:  Determined  by  its
ability  to  chemoattract  human  T  cells
using a concentration  range of 2.0-10.0
ng/ml.
 
Protein Sequence:  
MKFISTSLLLMLLVSSLSPVQGVLEVYY
TSLRCRCVQESSVFIPRRFIDRIQILPR
GNGC
PRKEIIVWKKNKSIVCVDPQAEWIQRM
MEVLRKRSSSTLPVPVFKRKIP 

References:
1.   Neurology,  Dec  2009;  73:  2003  -
     2010.
2.   Nephrol.  Dial.  Transplant.,  Dec
     2009; 24: 3708 - 3712.