FGF2/bFGF (human, recombinant)
Catalog #: 90300-4

Size: 50 µg

Description: Recombinant human soluble basic FGF (GenBank Accession No.
NM_002006) is a 17.3 kDa (134 aa-288 aa) protein expressed in an E.coli expression
system.

Source: E.coli

Formulation: 5 mM Tris, pH 7.5, 150 mM NaCl.

Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -
20°C.

Purity: >98% by SDS-PAGE and HPLC analysis.

Endotoxin Level: < 1EU per ug of bFGF as determined by the LAL method.

Biological Activity: Measured by its ability to stimulate proliferation of NIH3T3 cells.
The ED50 of this effect is between 0.1 - 1 ng/ml.

References:
1. Derivation of pluripotential embryonic stem cells from murine primordial germ cells in
culture. Cell 70(5), 841-847 (1992).
2. Differentiation of human embryonic stem cells into insulin-producing clusters. Stem
Cells. 22(3), 265-74 (2002).