BAFF-R, Human BAFF-R(CD268) Recombinant
Catalog # 90103-B
Size: 50 µg

Description:  Recombinant  human
BAFF-R (B cell-activating factor from the
TNF family), also known as CD268, is a
disulfide-linked  monomeric  protein
consisting  of  76  amino  acids  and
migrates  as  an  approximately  9  kDa
protein  under  reducing  and  non-reducing
conditions.  

Source:  Optimized  DNA  sequence
encoding  human  BAFF-R  extracellular
domain was expressed in E. coli.

Formulation: Lyophilized from a 0.2 μm
filtered solution containing 2.5% glycine,
0.5%  sucrose,  0.01% Tween-80,  5 mM
glutamic acid, pH 4.5.

Reconstitution:  Reconstitute at 0.1-1.0
mg/mL  in  distilled  water.  This  solution
can then be diluted into other buffers. To
maximize  product  collection  from  vial
surface,  vortex  briefly  and  then  spin
down to recollect the liquid. 

Storage:  The  lyophilized  protein  is
stable  for  at  least  2  years  from  date  of
receipt  when  stored  at  -20°C. Upon
reconstitution,  store  in working  aliquots
at 2  - 8° C  for up  to one month, or at  -
20°C  for  up  to  six  months,  in  the
presence  of  a  carrier  protein. Avoid
repeated freeze/thaw cycles.

Purity:  >95%,  as  determined  by  SDSPAGE
 and HPLC.

Endotoxin  Level:  Endotoxin  level  was
found to be < 0.1 ng/μg (1EU/μg), using
the LAL gel clot method.
 
Biological  Activity:  The  ED50,
determined by  the ability  to block BAFF
induced survival of splenocyte cells, was
found  to  be  in  the  range  of  1.0-5.0
ug/ml.
 
Protein Sequence: 
MRRGPRSLRGRDAPAPTPCVPAECF
DLLVRHCVACGLLRTPRPKPAGASSP
APRTALQPQESVGAGAGEAALPLPGL
L
 
References:
1.   Blood, Dec 2009; 114: 5362-67.
2.   Blood  (ASH  Annual  Meeting
     Abstracts), Nov 2009; 114: 2400.