aFGF (mouse), Mouse acidic Fibroblast Growth Factor Recombinant
Catalog # 90102-B
Size: 50 µg

Description:  Recombinant  murine
aFGF  (acidic  Fibroblast Growth  Factor)
is  a  disulfide-linked  monomer  protein
consisting  of  141  amino  acids  and
migrates  as  an  approximately  16  kDa
protein  under  reducing  and  non-reducing
conditions. Native  murine aFGF,  generated 
by  the  proteolytic removal  of  the  signal 
peptide  and propeptide, has a calculated
mass of 16 kDa. 

Source:  Optimized  DNA  sequence
encoding  murine  acidic  Fibroblast
Growth  Factor  mature  chain  was
expressed in E. coli.

Formulation: Lyophilized from a 0.2 μm
filtered solution containing 2.5% glycine,
0.5%  sucrose,  0.01% Tween-80,  5 mM
glutamic acid, pH 4.5.

Reconstitution:  Reconstitute at 0.1-1.0
mg/mL  in  distilled  water.  This  solution
can then be diluted into other buffers. To
maximize  product  collection  from  vial
surface,  vortex  briefly  and  then  spin
down to recollect the liquid. 

Storage:  The  lyophilized  protein  is
stable  for  at  least  2  years  from  date  of
receipt  when  stored  at  -20°C.  Upon
reconstitution,  store  in working  aliquots
at  2  -  8°C  for  up  to  one  month,  or  at      
-20°C  for  up  to  six  months,  in  the
presence  of  a  carrier  protein.  Avoid
repeated freeze/thaw cycles. 

Purity:  >95%,  as  determined  by  SDS-
PAGE and HPLC.

Endotoxin  Level:  Endotoxin  level  was
found to be < 0.1 ng/μg (1EU/μg), using
the LAL gel clot method.
 
Biological  Activity:  The  ED50  as
determined  by  the  dose-dependent
stimulation of thymidine uptake by BaF3
cells  expressing FGF  receptors  is  <  10
ng/ml,  corresponding  to  a  specific
activity of ≥1 x 10⁵ units/mg.
 
Protein Sequence: 
FNLPLGNYKKPKLLYCSNGGHFLRILP
DGTVDGTRDRSDQHIQLQLSAESAGE
VYIKGTETGQYLAMDTEGLLYGSQTP
NEECLFLERLEENHYNTYTSKKHAEKN
WFVGLKKNGSCKRGPRTHYGQKAILF
LPLPVSSD
 
References:
1.   J. Clin. Pathol., Jan 2001; 54: 37 -
     41.
2.   J. Biol. Chem., Apr 2000; 275:
     11972.