BRD2 (339-459)
Human, recombinant, N-terminal His-tag

Catalog #: 31020
Size: 100 µg

Description: Human Bromodomain containing 2, also
known as BRD2, RING3 or RNF3, amino-acids 339 - 459 (GenBank Accession No. NM_005104) with N-terminal HIS tag, MW = 15.1 kDa, expressed in an E. coli expression system.

Assay Conditions: BRD2 protein (200
ng/μl) is incubated with biotinylated acetylhistone
peptide substrate (Cat. #33000) in
BRD assay buffer (Cat. #33001) in a 10 ul
reaction for 1 hour at RT. AlphaLISA
(R) GSH acceptor beads (Perkin Elmer) are
added, followed by AlphaScreen(R)
Streptavidin-conjugated donor beads (Perkin
Elmer). After 15 minute incubation at RT,
Alphacounts are measured.

Application: Useful for the study of bromodomain
binding assays, screening inhibitors and selectivity 
profiling.

Formulated in: 50 mM Tris-HCl, pH 8.0,
138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 
and 10% glycerol.

Stability: >6 months at -80°C

References:
1. Umehara, T., et al., FEBS Lett. 2010

Sep 24; 584(18): 3901-3908.
2.  Shang, E., et al., J Cell Biochem. 2011
Oct; 112(10): 2784-2793.

PRODUCT DATA SHEETS
Lot 120207G

Data shown is lot-specific. For specific information on other lots, please contact us.