Anti-RFP Tag
Mouse Monoclonal Antibody

Catalog #: 25013

Size: 100 μg

Clonality | Host Species | Isotype:
RF5R | Mouse | IgG1

Formulation:
1mg/mL in 10mM PBS, pH 7.2, with 0.05%
NaN3

Sensitivity:
WB (with ECL): 1-10ng of purified RFP or
RFP fusion proteins

Working Conditions:
WB (with ECL): 1:1000-3000 dilution
(incubate for 1 hour at room temperature)
ICC: 1:500-2000 dilution
In order to obtain best results in other
assays (e.g.: Dot Blot, ELISA, IP, etc.),
please determine optimal working dilution by
titration test

Purification:
Protein A affinity chromatography from
mouse ascites fluid

Storage and Stability:
Store at -20°C for up to 2 years. Centrifuge
after first thaw to maximize product
recovery. Aliquot to avoid repeated freezethaw
cycles. Store aliquots at 4°C for 1-2
weeks

Specificity:
Recognizes native and denatured forms
of RFP and its variants: tag-RFP, turbo-
RFP, DeRed, mCherry, mOrange, etc.

Immunogen:
N-terminal peptide from RFP (Discosoma
sea anemone)- KLH conjugate

Applications:
Dot Blot, ELISA, IP, ICC, WB

Click here to download the product data sheet.