Anti-GST Tag (GST.B6/G2R)
Mouse Monoclonal Antibody
Catalog #: 25009
Size: 100 µg
Clonality | Host Species | Isotype:
GST.B6/G2R | Mouse | IgG2a
Formulation:
1mg/mL in 10mM PBS, pH 7.2, with 0.05%
NaN3
Sensitivity:
WB (with ECL): 10-50ng of purified GST or
GST fusion proteins
Working Conditions:
WB (with ECL) 1:1000-3000 dilution
(incubate for 1 hour at room temperature)
ICC: 1:500-2000 dilution
For best results with other assays (e.g.: Dot
Blot, ELISA, IP, etc.), please determine
optimal working dilution by titration test
Purification:
Protein A affinity chromatography from
mouse ascites fluid
Storage and Stability:
Store at -20°C for up to 2 years. Centrifuge
after first thaw to maximize product
recovery. Aliquot to avoid repeated freezethaw
cycles. Store aliquots at 4°C for 1-2
Specificity:
Recognizes native and denatured forms
of purified GST or GST fusion proteins
Immunogen:
Recombinant Glutathione-S-transferase
protein (S. japonicum)
Applications:
Dot Blot, ELISA, ICC, IP, WB