Anti-GAPDH (GA1R)
Mouse Monoclonal Antibody
Catalog #: 25007
Size: 100 μg
Clonality | Host Species | Isotype:
GA1R | Mouse | IgG1
Formulation:
1mg/mL in 10mM PBS, pH 7.2, with 0.05%
NaN3
Cross Reactivity:
GAPDH from BL-21 bacteria, Sf9 insect,
Saccharomyces cerevisiae (yeast), human,
mouse, rat, rabbit, chicken, and hamster.
GAPDH from other species may also be
detectable
Working Conditions:
WB (with ECL): 1:1000-100,000 dilution
(incubate for 1 hour at room temperature)
ICC: 1:500-2000 dilution
For best results with other assays (e.g.: Dot
Blot, ELISA, etc.), please determine optimal
working dilution by titration test
Purification:
Protein A affinity chromatography from
mouse ascites fluid
Storage and Stability:
Store at -20°C for up to 2 years. Centrifuge
after first thaw to maximize product
recovery. Aliquot to avoid repeated freezethaw
cycles. Store aliquots at 4°C for 1-2
weeks
Specificity:
Recognizes native and denatured forms
of GAPDH (~37kDa)
Immunogen:
Recombinant GAPDH
Applications:
Dot Blot, ELISA, ICC, WB