Anti-FLAG Tag (FG4R)
Mouse Monoclonal Antibody

Catalog #: 25003

Size: 100 µg

Clonality | Host Species | Isotype:
FG4R | Mouse | IgG2b

Formulation:
1mg/mL in 10mM PBS, pH 7.2, with 0.05%
NaN3

Sensitivity:
WB (with ECL): 1-5ng of FLAG-tagged
fusion proteins

Working Conditions:
WB (with ECL): 1:1000-3000 dilution
(incubate for one hour at room temperature)
ICC: 1:500-2000 dilution
For best results with other assays (e.g.: Dot
Blot, ELISA, IP, etc.), please determine
optimal working dilution by titration test

Purification:
Protein A affinity chromatography from
mouse ascites fluid

Storage:
Store at -20°C for up to 2 years. Centrifuge
after first thaw to maximize product
recovery. Aliquot to avoid repeated freeze-
thaw cycles. Store aliquots at 4°C for 1-2
weeks

Specificity:
Recognizes the N-terminal, C-terminal or
internal FLAG-tagged fusion proteins

Immunogen:
DYKDDDDK (FLAG) synthetic peptide
conjugated to KLH

Applications:
Dot Blot, ELISA, IP, ICC, WB

Click here to download the product data sheet