Anti-ERK1 (E31)
Mouse Monoclonal Antibody
Catalog #: 25005
Size: 100 μg
Clonality | Host Species | Isotype:
E32 | Mouse | IgG1
Formulation:
1mg/mL in 10mM PBS, pH 7.2, with 0.05%
NaN3
Cross Reactivity:
WB: ERK1 from human and mouse cells. IP:
ERK1 from human cells. ERK1 from other
species may also be detectable.
Does not recognize ERK2 protein.
Working Conditions:
WB (with ECL): 1:1000-10,000 dilution
(incubate for one hour at room temperature)
For best results with other assays (e.g.: IP,
etc.), please determine optimal working
dilution by titration test
Purification:
Protein A affinity chromatography from
mouse ascites fluid
Storage:
Store at -20°C for up to 2 years. Centrifuge
after first thaw to maximize product
recovery. Aliquot to avoid repeated freezethaw
cycles. Store aliquots at 4°C for 1-2
weeks
Specificity:
Recognizes only the ERK1 protein
Immunogen:
Recombinant full length ERK1 protein
Applications:
IP, WB