Anti-Claudin-18 Isoform 2 Antibody, PE-Labeled

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Catalog #

101676

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Description

This anti-Claudin-18 isoform 2 (CLDN18.2) antibody is a purified recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested in flow cytometry assay for specific binding to human CLDN18.2 on the surface of CLDN18.2 CHO Recombinant Cell Line (BPS Bioscience #78533).

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Synonyms

Claudin-18, CLDN18, UNQ778, PRO1572

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Product Data Gallery

Cell surface expression of CLDN18.2 in CLDN18.2 CHO Cell Line

Product Info

Storage and Usage

Citations

Species

Human

Isotype

IgG1

Clonality

Monoclonal

Host Species/Expression System

HEK293

Purity

≥90%

Purification

Protein A affinity chromatography from HEK293 supernatants.

Format

Aqueous buffer solution.

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, 0.09% Sodium Azide, 0.2% BSA, and up to 20% glycerol. May contain protein stabilizer.

Concentration

The concentration will vary with each lot; the exact value will be provided with each shipment.

UniProt #

P56856

Label

PE is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with excitation peak at 566 nm and an emission peak at 574 nm. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa).

Storage/Stability

At least 6 months at 4°C. Protect from prolonged exposure to light. DO NOT FREEZE. The antibody solution should be stored undiluted between 2°C and 8°C.

Assay Conditions

One million CLDN18.2 CHO recombinant cells (BPS Bioscience #78533) were blocked and stained with 1 µg of anti-CLDN18.2 antibody, PE-labeled (BPS Bioscience #101676) for 30 minutes on ice, washed three times, and analyzed by flow cytometry. CLDN18.2 CHO recombinant cells (green) were compared to parental CHO-K1 cells (blue). Y-axis is the cell count. X-axis is PE intensity.

Applications

Useful for studying expression of CLDN18.2 on the cell surface in flow cytometry assay. For flow cytometric staining, the suggested use of this reagent is 5 µl (1 µg antibody) per million cells in 100 µl staining volume.

Shipping Temperature

+4°C (gel packs)

Cross Reactivity

This antibody recognizes human CLDN 18.2. It has not been tested with other species.

Warnings

Do not freeze. Protect from light.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Anti-Claudin-18 Isoform 2 Antibody, PE-Labeled

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