Fluorogenic P300 Assay Kit
Catalog #: 50092

Size: 96 reactions

DESCRIPTION:  The Fluorogenic p300 Assay Kit is a homogeneous, fluorogenic assay designed to screen for inhibitors of p300 or to measure histone acetyltransferase (HAT) activity using cell extracts or purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HAT activity measurements. No time-consuming washing steps are required.  In addition, the kit includes purified p300 (also known as KAT3B) for use as a positive control.  

The Fluorogenic p300 Assay Kit is based on the transfer of an acetyl group from acetyl CoA to a peptide substrate.  After incubation with acetyl CoA and the substrate, the P300 enzyme generates acetylated H3 peptide and CoASH. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation = 360 nm and emission = 460 nm.

COMPONENTS:
 

APPLICATIONS: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

CONTRAINDICATIONS: DTT, β-mercaptoethanol, or other reducing reagents, DMSO >1%, strong acids or bases, ionic detergents, high salt.

REFERENCE:
Trievel, R. C., et al. (2000). Anal. Biochem. 287(2):319-28.

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